Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer
نویسندگان
چکیده
منابع مشابه
Production of Transgenic Wuzhishan Inbred Miniature Pigs Carrying Green Fluorescent Protein by Somatic Cell Nuclear Transfer
In this study, based on somatic cell nuclear transfer (SCNT), we used the ear fibroblasts of miniature pigs transfected with enhanced green fluorescent protein (EGFP) as the donor cells and transplanted reconstructed embryos (at the 1–8 cell stage) into the oviducts of three sexually mature Wuzhishan miniature pigs that were naturally estrus. One recipient successfully birthed two stillborn and...
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Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
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Somatic cell nuclear transfer (SCNT) offers great potential for developing better animal models of human disease. The domestic ferret (Mustela putorius furo) is an ideal animal model for influenza infections and potentially other human respiratory diseases such as cystic fibrosis, where mouse models have failed to reproduce the human disease phenotype. Here, we report the successful production ...
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The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have either been inefficient or not available in all animals. More recently, somatic cell nuclear transfer has provided a method to create transgenic a...
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Among its many functions, the ubiquitin-proteasome system regulates substrate-specific proteolysis during the cell cycle, apoptosis, and fertilization and in pathologies such as Alzheimer's disease, cancer, and liver cirrhosis. Proteasomes are present in human and boar spermatozoa, but little is known about the interactions of proteasomal subunits with other sperm proteins or structures. We hav...
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ژورنال
عنوان ژورنال: Science Bulletin
سال: 2008
ISSN: 2095-9273,2095-9281
DOI: 10.1007/s11434-008-0168-x